Robust PCR amplifications with low error rate
Key Features
- Low error rate of only 4 nucleotides per 1 million nucleotide incorporation events.
- Generate amplicons over 10 kb on genomic templates or 40 kb on less complex DNA.
- Direct replacement for more expensive high fidelity polymerases.
Description
A unique blend of SharkaTAQ DNA Polymerase & a proof-reading polymerase with 3' to 5' exonuclease activity.
Performance
SharkaTAQ outperforms competitive enzymes but at a fraction of the cost.
Applications
- Use for sequencing, cloning and TILLING applications or in any situation where low error rate is desirable.
Kit Components
- SharkaTAQ Hot Start DNA Polymerase + proof-reading polymerase 5 Units / ul.
- 10 x reaction buffer containing 18mM MgCl2 + detergent.
- Separate tube of 50mM MgCl2 for assay optimization.
Ordering Information
- 500 Units: $350
- 5,000 Units: $3,500
- 50,000 Units: $30,000
Contact us for bulk discounting, institutional pricing & custom packaging. Also available as 2x Master Mix.
Storage & Stability
- Stable for at least one year at -20°C.
- Avoid repeated freeze-thaw cycles.
- Aliquot into separate tubes and store at –20°C.
Usage restrictions
All products are intended to be used for research purposes only. They are not to be used for drug or diagnostic purposes nor are they intended for human use.
Frontier Genomics products may not be re-sold or transferred, modified for re-sale or transfer or used to manufacture commercial products without written approval from Frontier Genomics LLC.