Robust PCR with low error rate in a convenient pre-mixed formulation supplied at 2x concentration

Key Features

2x Master Mix format – just add primers, template and H₂O.
Low error rate of only 4 nucleotides per 1 million nucleotide incorporation events.
Increased reproducibility and throughput, decreased risks of contamination.
Generate amplicons over 10 kb on genomic templates or 40 kb on less complex DNA.
Robust enzyme ideal for any PCR application requiring low error rate.
Fast, easy assembly at room temperature.
Direct replacement for more expensive Hot Start high fidelity master mix kits.

Description

A unique blend of SharkaTAQ Hot Start DNA Polymerase & a proof-reading polymerase with 3' to 5' exonuclease activity supplied in a convenient pre-mixed formulation at 2 x concentration. Simply add primers, template & H₂O.

Performance

Gel comparing SharkaTAQ DNA polymerase with leading competitor

SharkaTAQ outperforms competitive enzymes but at a fraction of the cost.

Applications

Use for sequencing and cloning applications or in any situation where low error rate is desirable.

Kit Components

SharkaTAQ Hot Start DNA Polymerase & a proof-reading polymerase with 3' to 5' exonuclease activity in a pre-mixed formulation at 2 x concentration. Simply add primers, template & H₂O.

Ordering Information

Price:

100 x 50ul reactions (2.5mls): $150
200 x 50ul reactions (5mls): $300
1000 x 50ul reactions (25mls): $1,500

Storage & Stability

Stable for at least one year at -20°C.
Avoid repeated freeze-thaw cycles.
Aliquot into separate tubes and store at –20°C.
Storage at 4°C is recommended only for periods of up to 1 week.

Usage restrictions

All products are intended to be used for research purposes only. They are not to be used for drug or diagnostic purposes nor are they intended for human use.

Frontier Genomics products may not be re-sold or transferred, modified for re-sale or transfer or used to manufacture commercial products without written approval from Frontier Genomics LLC.