High specificity, yield and sensitivity for use in everyday PCR applications
Key Features
- High specificity reduces potential for background amplifications.
- Robust enzyme ideal for many PCR applications.
- Easy assembly and room temperature set up.
- Direct replacement for more expensive Hot Start Taq polymerases.
Description
SharkaTAQ Hot Start DNA Polymerase is supplied in an inactive state that has no polymerase activity at ambient temperatures due to a novel patent pending method. This prevents non specific amplification resulting from extension of nonspecifically annealed primers and primer–dimers formed at low temperatures during PCR setup and the initial PCR cycle. Provides high specificity, yield and sensitivity and is ideal for use in everyday PCR applications.
Performance
SharkaTAQ outperforms competitive enzymes but at a fraction of the cost.
Applications
- Any PCR situation that requires high specificity, yield or sensitivity.
- Multiplex PCR (reactions with multiple primers).
- Complex genomic templates.
- Complex cDNA templates e.g RT-PCR.
- Very low-copy targets e.g. single-cell PCR.
Kit Components
- SharkaTAQ Hot Start DNA Polymerase 5 Units / ul
- 10 x PCR Buffer A and 10 x PCR Buffer B
- Buffer A – use when yield is most important
- Buffer B – use when specificity is most important
- Includes Mg for further assay optimization & DMSO for GC rich sequences
Ordering Information
Price:
- 500 Units: $300
- 5,000 Units: $2,250
- 50,000 Units: $20,000
Contact us for bulk discounting, institutional pricing & custom packaging. Also available as 2x Master Mix.
Storage & Stability
- Stable for at least one year at -20°C.
- Avoid repeated freeze-thaw cycles.
- Aliquot into separate tubes and store at –20°C.
Usage restrictions
All products are intended to be used for research purposes only. They are not to be used for drug or diagnostic purposes nor are they intended for human use.
Frontier Genomics products may not be re-sold or transferred, modified for re-sale or transfer or used to manufacture commercial products without written approval from Frontier Genomics LLC.