products and can identify single mutants within a pool of samples to increase throughput for TILLING and reduce ascertainment bias for
EcoTILLING
OPTIMIZED REAGENTS FOR THE LOW COST & RAPID DISCOVERY OF USEFUL SNPs IN ANY SPECIES
TILLING and EcoTILLING applications were originally designed to be used on the LICOR® DNA Analyzer, but have moved to numerous other platforms which do not require the use of dye labeled primers. The benefits are an inexpensive platform for reverse genetics and rapid SNP discovery, which still allows pooling of samples to increase throughput and reduce discovery bias. TILLING-U™ kits contain SNiPerase-U™, which was specifically developed for optimal cleaveage of unlabeled PCR fragments.
TILLING studies need to survey target PCR fragments in thousands, tens of thousands or hundreds of thousands of individuals to identify induced mutations correlated with a desired phenotype.
EcoTILLING studies are primarily concerned with identifying informative SNPs for population genetics, forensics, conservation and resource management work. Inclusion of too few individuals in the discovery panel can introduce ascertainment bias. EcoTILLING allows hundreds or thousands of individuals to be included in the discovery panel by pooling, which reduces ascertainment bias, and allows for the discovery of the most informative SNPs for the study at hand.
The TILLING-U kit™ includes reagents for PCR amplification of the target PCR fragment, cleavage of the heteroduplexes, halting of the reaction, and heterozygous and homozygous controls to troubleshoot.
Discounts and custom packaging available for bulk orders. Please contact us for more information.
Key Benefits
- Optimized for systems that require unlabeled primers
- Comes complete with control kit for troubleshooting
- Includes SharkaTAQ™ DNA polymerase and dNTPs
- Contains all reagents to perform PCR and cleavage reactions with one optimized kit
- Includes both a heterozygous and a homozygous control mix containing LI-COR IRDye® primers and template
Ordering Information
- 1000 reactions: $750
Contact us for bulk discounting, institutional pricing & custom packaging.
Contents
- SharkaTAQ™ DNA polymerase (MSDS, PDF)
- SNiPerase-U™ nucleases (Optimal mix of cleavage enzymes) (MSDS, PDF)
- DNA Control Primers (MSDS)
- MgCl2 (MSDS, PDF)
- dNTPs (MSDS, PDF)